Pour the mixture of lysate and Ni‐NTA agarose in to the column, and permit it to empty both by gravity circulation or by applying a vacuum to The underside with the column. This short article is being made freely available as a result of PubMed Central as Element of the https://www.facebook.com/NewTechFind/posts/pfbid032TPHc3JdtuJHT3gVQGc64WsvMnEpTWf7uC3nfkG55ojbcdzZaTURpZ191tmpeuPTl