Recurring cycles of amplification lead to degradation of your probe, leading to greater fluorescence. TaqMan detection is, thus, far more precise than dye‐centered detection, because an increase in fluorescence occurs only on binding from the probe to a specific sequence and subsequent de‐quenching, although no signal is made by amplification https://www.facebook.com/NewTechFind/posts/pfbid0YPAzGvVz72mKah68ctQg3wP1JFbs9jq9dyoRfz9AkydHS5u7wshhDSMEEcCg7NQCl